Nanoparticles, due to their exceptionally high surface area and geometric structure, can significantly enhance anti-wear properties and reduce friction when combined with lubricants. This research consists of three stages. In the first stage, nanoparticles were produced from sugarcane bagasse (nano-biochar) using mechanical milling for durations of 4 and 8 hours. The production of biochar nanoparticles from sugarcane waste as bio-nanoparticles can be a step toward mitigating environmental issues. The nanoparticles produced at these two durations were evaluated using EDS and XRD tests, and based on the results, the nanoparticles produced after 8 hours were deemed more suitable.In the second stage, the nanoparticles were mixed with Pars Super Kian 6000 oil, commonly used in motorcycles, at concentrations of 0.5%, 1%, and 2% by weight. The physical and thermal properties of each mixture were determined. Based on the conducted tests and the results obtained for the four oil samples, it was found that the nano-lubricant with a 2% weight concentration exhibited the best performance. These results indicate that increasing the weight concentration of biochar nanoparticles as an additive in the oil has a positive effect on improving its physical and thermal properties.