Nanofluids are a new generation of heat transfer fluid, which are produced by the uniform and stable dispersion of nano particles in a base fluid. Nanofluids containing solid nanoparticles have a higher viscosity than conventional working fluids. This characteristic of nanofluids has attracted the attention of many researchers. In this study, we have experimentally measured the dynamic viscosity of Ethylene Glycol- Zinc oxide nanofluid and presented the new governing correlation. In order to prepare Ethylene Glycol-Zinc oxide nanofluid, the two-step suspension method was used by ultrasonic mixer. For this purpose, nanoparticles with diameter of 20 nm in volume fractions of 0.2, 0.4, 0.6, 0.8 and 1% were used at temperatures of 20, 25, 30, 35, 40, 45 and 50 ° C. Initially, the Brookfield DV3-TLV viscometer was used to measure dynamic viscosity in different volume fractions, and then using the experimental results, a two-variable experimental correlation was presented with linear regression with R² = 0.993 in order to estimate dynamic viscosity by considering the effects of volume fraction of nanoparticle and nanofluid temperature.